Excessive fluoride increases the expression of osteocalcin in the mouse testis / 中华男科学杂志
National Journal of Andrology
;
(12): 782-785, 2017.
Article
in Chinese
| WPRIM
| ID: wpr-812879
ABSTRACT
Objective@#To observe the influence of excessive fluoride on the levels of osteocalcin and testosterone in the testis of the male mouse.@*METHODS@#Twenty-four C57BL/6J male mice were equally randomized into a normal control and a fluorosis model group, the former fed on distilled water while the latter on a solution of sodium fluoride (100 mg/L) in distilled water, both for 12 weeks. Then, the level of osteocalcin in the testis tissue was measured with the immunohistochemical streptavidin-peroxidase (SP) method and those of osteocalcin and testosterone in the serum determined by ELISA.@*RESULTS@#After 12 weeks of fluoride intervention, the level of serum osteocalcin was significantly higher in the fluorosis models than in the normal controls ([68.05 ± 5.32] vs [47.50 ± 5.73] pg/mL, F = 11.901, P = 0.008), while that of testosterone markedly lower in the former than the latter group ([8.07 ± 1.35] vs [12.94 ± 3.09] ng/mL, F = 2.313, P = 0.006). The results of immunohistochemical SP showed the expression of osteocalcin in the cell membrane and cytoplasm of the fluorosis models, which was evidently higher than in the normal controls.@*CONCLUSIONS@#Twelve-week intake of 100 mg/L fluoride solution can decrease the level of testosterone and increase the expression of osteocalcin in the testis of the male mouse.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Sodium Fluoride
/
Testis
/
Random Allocation
/
Osteocalcin
/
Toxicity
/
Fluoride Poisoning
/
Fluorides
/
Metabolism
/
Mice, Inbred C57BL
Limits:
Animals
Language:
Chinese
Journal:
National Journal of Andrology
Year:
2017
Type:
Article
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