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P38 MAPK inhibitor SB203580 attenuates the toxicity of ropivacaine on PC12 cells / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences) ; (12): 985-989, 2019.
Article in Chinese | WPRIM | ID: wpr-813060
ABSTRACT
To investigate the effect of SB203580, a p38MAPK specific inhibitor, on ropivacaine-induced cytotoxicity in PC12 cells.


Methods:

PC12 cells were divided into three groups the normal group (Group N), cells were cultured for 48 h; the ropivacaine group (Group R), cells were cultured with 15 mmol/L ropivacaine hydrochloride for 48 h; the ropivacaine+SB203580 group (Group R+S), cells were cultured with 15 mmol/L ropivacaine hydrochloride plus 10 μmol/L SB203580 for 48 h. The cell survival rates were detected by MTT assay. The protein levels of cleaved caspase-3, phosphor-p38 (p-p38) and cystolic cytochrome C (Cyt C) were detected by Western blotting.


Results:

Compared with the Group N, the number and survival rate of PC12 cells in the Group R and the Group R+S were significantly reduced (all P<0.05); the number and survival rate of PC12 cells in the Group R+S were significantly higher than those in the Group R (both P<0.05). Compared with the Group N, the levels of p-p38 and cleaved caspase-3, and the content of cytoplasmic Cyt C in the PC12 cells from the Group R and the Group R+S were significantly enhanced (all P<0.05); compared with the Group R, the levels of p-p38 and cleaved caspase-3, and the content of cytoplasmic Cyt C in the PC12 cells from the Group R+S were decreased (all P<0.05).


Conclusion:

The ropivacaine-induced cytotoxicity can be attenuated via inhibition of p38MAPK; which is related to decrease in Cyt C content and cleaved caspase-3 expression.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pyridines / PC12 Cells / Apoptosis / P38 Mitogen-Activated Protein Kinases / Toxicity / Ropivacaine / Imidazoles / Anesthetics, Local / Metabolism Limits: Animals Language: Chinese Journal: Journal of Central South University(Medical Sciences) Year: 2019 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pyridines / PC12 Cells / Apoptosis / P38 Mitogen-Activated Protein Kinases / Toxicity / Ropivacaine / Imidazoles / Anesthetics, Local / Metabolism Limits: Animals Language: Chinese Journal: Journal of Central South University(Medical Sciences) Year: 2019 Type: Article