Effect of ursolic acid on invasion and migration of hepatocellular carcinoma cells co-cultured with macrophages and the underlying mechanisms / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
; (12): 1188-1193, 2018.
Article
in Zh
| WPRIM
| ID: wpr-813117
Responsible library:
WPRO
ABSTRACT
To investigate the effect of ursolic acid on the invasion and migration of hepatocellular carcinoma (HCC) cells co-cultured with macrophages, and to explore the underlying mechanisms.
Methods: The migration and invasion ability of HCC cells in the co-culture system with or without ursolic acid intervention were evaluated by transwell assay. The levels of epithelial-mesenchymal transition (EMT) markers E-cadherin, N-cadherin, and vimentin in HCC cells co-cultured with macrophages were detected by Western blot.
Results: The migration and invasion ability and EMT were significantly enhanced when co-cultured with macrophages, and the expression of E-cadherin was significantly increased while N-cadherin and vimentin levels were significantly decreased. However, after ursolic acid treatment, the migration and invasion ability were significantly reduced, and the expression of E-cadherin was increased while N-cadherin and vimentin levels were decreased.
Conclusion: Ursolic acid exerts inhibitory effect on the ability of migration, invasion, and EMT for HCC, which are enhanced by co-culturing with macrophages.
Full text:
1
Index:
WPRIM
Main subject:
Pathology
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Pharmacology
/
Triterpenes
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Cadherins
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Gene Expression Regulation, Neoplastic
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Cell Movement
/
Carcinoma, Hepatocellular
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Coculture Techniques
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Cell Biology
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Cell Line, Tumor
Limits:
Humans
Language:
Zh
Journal:
Journal of Central South University(Medical Sciences)
Year:
2018
Type:
Article