Vascular endothelial growth factor antibody attenuates diabetic peripheral neuropathic pain in rats / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 1097-1102, 2018.
Article
in Chinese
| WPRIM
| ID: wpr-813148
ABSTRACT
To explore the role of vascular endothelial growth factor (VEGF) in diabetic peripheral neuropathic pain in rats.
Methods:
Twenty-four adult male Sprague-Dawley rats aged 8 weeks were randomly divided into 3 groups (n=8 per group). The control group (C group) rats were intraperitoneally injected with sodium citrate solution at 10 mL/kg; the model group (M group) rats were intraperitoneally injected with streptozotocin at 65 mg/kg; the treatment group (T group) rats received intraperitoneal injection of anti-VEGF antibody (10 mg/kg) at the 1st, 3rd, 7th, 10th day after STZ treatment. Meanwhile, rats of C and M group were received with the same volume of sodium citrate solution. Blood glucose was measured before 1 day or at the 1st, 3rd, 7th or 14th day after receiving STZ. Body weight, paw withdrawal mechanical threshold (PWMT) and paw withdrawal thermal latency (PWTL) were measured before 1 day or at the 1st, 3rd, 5th, 7th, 10th or 14th day after receiving STZ. All lumbar spinal cords were dissected to examine the p-protein kinase B (p-Akt) and transient receptor potential vanilloid 1 (TRPV1) expression by Western blot.Results:
After injection with STZ, the body weight showed significant differences at some time point between the M, T or C group (P<0.01); body weight of rat in the C group were increased gradually. Compared with the C group, the fast blood glucose in the M or the T Group at the same time points were increased significantly (P<0.01). The PWMT and PWTL of the M, T or C group were significant difference among various time points (P<0.01). The PWMT and PWTL in the M or T group were obviously reduced compared with those in the C group (P<0.01). Compared with the M group, the PWMT and PWTL in the T group were increased at the 10th or 14th day (P<0.01 or P<0.05). Compared with the C group, the p-Akt and TRPV1 levels in the M and T group were increased (P<0.01). Compared with the M group, p-Akt and TRPV1 levels in T group were decreased (P<0.01).Conclusion:
VEGF is able to regulate the expression of TRPV1 through PI3K/Akt pathway, which contributes to diabetic peripheral neuropathic pain in rats. Anti-VEGF treatment may be useful for alleviation of diabetic peripheral neuropathic pain.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Random Allocation
/
Gene Expression Regulation
/
Rats, Sprague-Dawley
/
Phosphatidylinositol 3-Kinases
/
Vascular Endothelial Growth Factor A
/
Therapeutic Uses
/
Diabetes Mellitus, Experimental
/
Diabetic Neuropathies
/
Drug Therapy
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Journal of Central South University(Medical Sciences)
Year:
2018
Type:
Article
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