Effect of JQ1 on expression of autoimmune-related genes in CD4+T cells of systemic lupus erythematosus / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 704-710, 2018.
Article
in Chinese
| WPRIM
| ID: wpr-813207
ABSTRACT
To investigate the effect of bromodomain and extra-terminal (BET) protein inhibitor JQ1 on expression of autoimmune-related genes in CD4+T cells from patients with systemic lupus erythematosus (SLE).
Methods:
Peripheral CD4+T cells were isolated by positive selection with CD4 microbeads. The percentage of CD4+T cells were detected by flow cytometry. CD4+T cells were treated by JQ1 at 100 nm/L for 6, 24, 48 h. The expression of T cell-related genes was measured by quantitative real-time PCR (qPCR). The secretion levels of cytokines in culture supernatant were measured by ELISA at 48 h.Results:
The percentage of CD4+T cells isolated by CD4 microbeads is 97.2%. Compared with the control group, the mRNA expression levels of IFNG, IL-17F, IL-21, CXCR5 and FOXP3 were down-regulated at 6, 24 and 48 h (P<0.05), and IL-17A mRNA level was decreased at 6 and 24 h (P<0.01); while IL-4 mRNA level was up-regulated at 24, 48 h (P<0.01), and TGF-β1 mRNA level was up-regulated at 6 and 48 h (P<0.05) in SLE CD4+T cells treated with JQ1. The secretion levels of IFN-γ and IL-21 in JQ1-treated group were decreased significantly (P<0.05), while the secretion levels of IL-4 and TGF-β were up-regulated compared with control group (P<0.05).Conclusion:
JQ1 can reverse the immune dysregulation and improve the immunity homeostasis in CD4+T cells from patients with SLE.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Azepines
/
Time Factors
/
Triazoles
/
RNA, Messenger
/
CD4-Positive T-Lymphocytes
/
Proteins
/
Cytokines
/
Interferon-gamma
/
CD4 Lymphocyte Count
Limits:
Humans
Language:
Chinese
Journal:
Journal of Central South University(Medical Sciences)
Year:
2018
Type:
Article
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