Effects of Nano-sized Carbon Black on the Lungs of High Fat-diet Induced Overweight Rats
Environmental Health and Toxicology
;
: e2013014-2013.
Article
in English
| WPRIM
| ID: wpr-81334
ABSTRACT
OBJECTIVES:
This study was conducted to determine whether nano-sized carbon black exposure results in greater damage in high fat diet-induced overweight rats than normal weight ones and to identify the possible causes of any differences.METHODS:
Two groups of Sprague-Dawley rats allocated by body weight (normal and overweight) were exposed to aerosolized nano-sized carbon black for 6 hours a day, 5 days per week over a 4-week period. Differential cell counts, lactate dehydrogenase (LDH) activities and albumin concentrations were measured in bronchoalveolar lavage (BAL) fluid, and histopathological findings in the lungs were evaluated. Tumor necrosis factor-alpha (TNF-alpha) and interleukin (IL)-6 were measured in BAL fluid and supernatants of lipopolysaccharide(LPS)-stimulated lymphocyte culture.RESULTS:
Rats exposed to high concentrations of nano-sized carbon black showed significantly increased (p<0.05) polymorphonuclear leukocyte number and LDH activity in the BAL fluid from both overweight and normal rats. Mild histopathological changes were observed in normal rats irrespective of carbon black concentrations. However, severe histological scores were found in overweight rats (1.75+/-0.46, 2.25+/-0.46, and 2.88+/-0.35 after low, medium, and high concentration exposures). Proinflammatory cytokine levels of TNF-alpha and IL-6 were significantly higher in the supernatant of LPS-stimulated lymphocytes of overweight rats, whereas there was no significant difference in the BAL fluid between normal and overweight rats.CONCLUSIONS:
Inflammation and damage to lungs exposed to nano-sized carbon black was more severe in high fat diet-induced overweight rats compared to normal rats.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Body Weight
/
Carbon
/
Lymphocytes
/
Cell Count
/
Inhalation
/
Interleukins
/
Interleukin-6
/
Tumor Necrosis Factor-alpha
/
Rats, Sprague-Dawley
/
Bronchoalveolar Lavage
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
Environmental Health and Toxicology
Year:
2013
Type:
Article
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