Effects of astragalus polysaccharides-chitosan/polylactic acid composite material on biological behavior of canine bone marrow stromal cells cultured in vitro / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
; (12): 283-287, 2005.
Article
in Zh
| WPRIM
| ID: wpr-813381
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To observe the biological behavior of canine bone marrow stromal cells (BMSCs) cultured in vitro with the astragalus polysaccharides-chitosan/polylactic acid (AP-C/PLA) and with the chitosan/polylactic acid (C/PLA) and to find a suitable compound material for periodontal tissue engineering.@*METHODS@#BMSCs (induced 14 days by 50 mg/L vitamine C, 10(-8) mol/L dexamethasone, 10 mmol/L beta-sodium glycerylphosphate) were cultured on AP-C/PLA or C/PLA for 5 days respectively. The BMSCs attachment and the morphology were observed with scanning electronic microscope and the combining rates were counted. Type I collagen synthesis was examined with immunohistochemistry staining and the content of osteocalin was determined with radio-immunological method.@*RESULTS@#Combining rates, type I collagen synthesis, and the content of osteocalin of BMSCs on AP-C/PLA were significantly higher than those on C/PLA.@*CONCLUSION@#AP-C/PLA may promote the BMSC proliferation, differentiation and extracellular matrix synthesis, and it can be used as a good scaffold material for bone tissue engineering.
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
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Polyesters
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Polymers
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Polysaccharides
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Bone Marrow Cells
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Drugs, Chinese Herbal
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Osteocalcin
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Cells, Cultured
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Stromal Cells
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Lactic Acid
Limits:
Animals
Language:
Zh
Journal:
Journal of Central South University(Medical Sciences)
Year:
2005
Type:
Article