Clone of hepatitis B virus X gene and its protein expression / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 525-528, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-813514
ABSTRACT
OBJECTIVE@#To construct the hepatitis B virus (HBV) X gene recombinant and to induce the expression of X protein.@*METHODS@#HBV DNA was extracted from the serum of patient with hepatitis B. The X gene was amplified by PCR using the primers with EcoRI and HindIII digestion sites, and then cloned into pronucleus expression vector pMAL-C2X, which was detected by EcoRI and HindIII digestion and sequence. Finally, the recombinant was induced by IPTG to express X protein in JM109.@*RESULTS@#The band similar to X gene was amplified by PCR. There were fragments similar to X gene when the recombinant was digested by the enzyme digestion. It was tested by DNA sequence that the correct and entire opening reading frame of HBV X gene was inserted. The X protein was expressed by the IPTG induction.@*CONCLUSION@#Pronucleus expression recombinant pMAL-C2X-HBV-X is constructed successfully and with the IPTG induction, the recombinant pMAL-C2X-HBV-X can express the X protein in E. coli JM109, which lays the foundation for the HBV X protein purification and its biological study.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Recombinant Proteins
/
Molecular Sequence Data
/
Base Sequence
/
Transfection
/
Trans-Activators
/
Hepatitis B virus
/
Open Reading Frames
/
Cloning, Molecular
/
Sequence Analysis, DNA
/
Escherichia coli
Limits:
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Journal of Central South University(Medical Sciences)
Year:
2005
Type:
Article
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