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Clone of hepatitis B virus X gene and its protein expression / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences) ; (12): 525-528, 2005.
Article in Chinese | WPRIM | ID: wpr-813514
ABSTRACT
OBJECTIVE@#To construct the hepatitis B virus (HBV) X gene recombinant and to induce the expression of X protein.@*METHODS@#HBV DNA was extracted from the serum of patient with hepatitis B. The X gene was amplified by PCR using the primers with EcoRI and HindIII digestion sites, and then cloned into pronucleus expression vector pMAL-C2X, which was detected by EcoRI and HindIII digestion and sequence. Finally, the recombinant was induced by IPTG to express X protein in JM109.@*RESULTS@#The band similar to X gene was amplified by PCR. There were fragments similar to X gene when the recombinant was digested by the enzyme digestion. It was tested by DNA sequence that the correct and entire opening reading frame of HBV X gene was inserted. The X protein was expressed by the IPTG induction.@*CONCLUSION@#Pronucleus expression recombinant pMAL-C2X-HBV-X is constructed successfully and with the IPTG induction, the recombinant pMAL-C2X-HBV-X can express the X protein in E. coli JM109, which lays the foundation for the HBV X protein purification and its biological study.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Recombinant Proteins / Molecular Sequence Data / Base Sequence / Transfection / Trans-Activators / Hepatitis B virus / Open Reading Frames / Cloning, Molecular / Sequence Analysis, DNA / Escherichia coli Limits: Female / Humans / Male Language: Chinese Journal: Journal of Central South University(Medical Sciences) Year: 2005 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Recombinant Proteins / Molecular Sequence Data / Base Sequence / Transfection / Trans-Activators / Hepatitis B virus / Open Reading Frames / Cloning, Molecular / Sequence Analysis, DNA / Escherichia coli Limits: Female / Humans / Male Language: Chinese Journal: Journal of Central South University(Medical Sciences) Year: 2005 Type: Article