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Cloning and Expression of the Gene Encoding the 32-kDa Protein of Rickettsia typhi
Journal of the Korean Society for Microbiology ; : 399-404, 1997.
Article in Korean | WPRIM | ID: wpr-81357
ABSTRACT
The crystalline surface layer protein (SLP) and a 28-32 kDa antigen of Rickettsia typhi were known as strong immunogens. We previously reported a cloning and sequence analysis of the SLP gene of R. typhi (slpT) and showed that the open reading frame of this gene encodes both the SLP and a 32-kDa protein. Our study also showed that a 48-kDa protein reacted strongly with polyclonal antiserum of a patient with murine typhus. In this study, we produced three recombinant proteins (SLP, 32-kDa, and 48-kDa protein) in E. coli as fusion proteins with maltose binding proteins. The reactivity of these proteins with patients' sera was investigated.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Rickettsia / Rickettsia typhi / Recombinant Proteins / Typhus, Endemic Flea-Borne / Open Reading Frames / Clone Cells / Sequence Analysis / Cloning, Organism / Crystallins / Maltose-Binding Proteins Limits: Humans Language: Korean Journal: Journal of the Korean Society for Microbiology Year: 1997 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Rickettsia / Rickettsia typhi / Recombinant Proteins / Typhus, Endemic Flea-Borne / Open Reading Frames / Clone Cells / Sequence Analysis / Cloning, Organism / Crystallins / Maltose-Binding Proteins Limits: Humans Language: Korean Journal: Journal of the Korean Society for Microbiology Year: 1997 Type: Article