Cloning and Expression of the Gene Encoding the 32-kDa Protein of Rickettsia typhi
Journal of the Korean Society for Microbiology
;
: 399-404, 1997.
Article
in Korean
| WPRIM
| ID: wpr-81357
ABSTRACT
The crystalline surface layer protein (SLP) and a 28-32 kDa antigen of Rickettsia typhi were known as strong immunogens. We previously reported a cloning and sequence analysis of the SLP gene of R. typhi (slpT) and showed that the open reading frame of this gene encodes both the SLP and a 32-kDa protein. Our study also showed that a 48-kDa protein reacted strongly with polyclonal antiserum of a patient with murine typhus. In this study, we produced three recombinant proteins (SLP, 32-kDa, and 48-kDa protein) in E. coli as fusion proteins with maltose binding proteins. The reactivity of these proteins with patients' sera was investigated.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Rickettsia
/
Rickettsia typhi
/
Recombinant Proteins
/
Typhus, Endemic Flea-Borne
/
Open Reading Frames
/
Clone Cells
/
Sequence Analysis
/
Cloning, Organism
/
Crystallins
/
Maltose-Binding Proteins
Limits:
Humans
Language:
Korean
Journal:
Journal of the Korean Society for Microbiology
Year:
1997
Type:
Article
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