Effect of chronic stress on PKA and P-CREB expression in hippocampus of rats and the antagonism of antidepressors / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 767-771, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-813602
ABSTRACT
OBJECTIVE@#To observe the effect of chronic unpredicted sequence of mild stress on the expression of cAMP-dependent protein kinase A(PKA) and phosphorylated cAMP-responsive element binding protein (P-CREB) in hippocampus of rats and the antagonism of antidepressors (fluoxetine).@*METHODS@#Thirty-six male Sprague Dawley rats were randomly and equally allocated to 3 groups A normal control group, a model group, and a fluoxetine group. All rats except the control group were singly housed and exposed to an unpredicted sequence of mild stressors. The different distribution and expression of PKA and P-CREB in the hippocampus of rats in different groups were investigated with immunohistochemistry and Westernblot technique.@*RESULTS@#The positive PKA and P-CREB cells in the hippocampus of normal controls were the pyramidal cells and the granule cells. The PKA and P-CREB protein expression levels in the hippocampus of model rats were significantly lower than those of the normal controls (P<0.05). The PKA and P-CREB protein expression levels in the hippocampus of the fluoxetine group were significantly higher than those of the model group (P<0.05).@*CONCLUSION@#Chronic unpredicted mild stress can affect the PKA and P-CREB expression in hippocampus of rats and fluoxetine has antagonism against it.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Stress, Physiological
/
Random Allocation
/
Fluoxetine
/
Rats, Sprague-Dawley
/
Cyclic AMP Response Element-Binding Protein
/
Cyclic AMP-Dependent Protein Kinases
/
Antidepressive Agents, Second-Generation
/
Depression
/
Genetics
/
Hippocampus
Limits:
Animals
Language:
Chinese
Journal:
Journal of Central South University(Medical Sciences)
Year:
2006
Type:
Article
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