Protective effect of resveratrol against oxidative damage of UVA irradiated HaCaT cells / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
; (12): 635-639, 2006.
Article
in En
| WPRIM
| ID: wpr-813631
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To observe the photoprotective effect and possible mechanisms of resveratrol for ultraviolet A (UVA) irradiated HaCaT cells.@*METHODS@#HaCaT cells under UVA irradiation with 5J/cm(2) were interfered with 0.01 mmol/L and 0.1 mmol/L resveratrol. The testing objects were divided into a control and a UVA irradiation group, and then we detected the proliferation capacity with methylthiazdyl tetrazolium (MTT) and superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) activity, content of maleic dialdehyde (MDA) with hydroxylamine, colorimetric, thiobarbituric acid (TBA) methods. The ultrastructure was observed under electron microscope.@*RESULTS@#Resveratrol could enhance the proliferation activity, SOD, GSH-Px activity of HaCaT cells under UVA irradiation, decrease the content of MDA in dose-dependent manner (P<0.05). The electron microscope revealed that resveratrol could relieve the injury of HaCaT cells' ultrastructure.@*CONCLUSION@#Resveratrol can relieve the inhibition to HaCaT cell proliferation,injury of their ultrastructure and oxidation by UVA irradiation. The protection is dose-dependent. That resveratrol raises the oxidase activity and clears the oxyradical may account for these results.
Full text:
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Index:
WPRIM
Main subject:
Pharmacology
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Radiation Effects
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Radiation-Protective Agents
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Stilbenes
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Superoxide Dismutase
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Ultraviolet Rays
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DNA Damage
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Cell Line, Transformed
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Keratinocytes
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Oxidative Stress
Limits:
Humans
Language:
En
Journal:
Journal of Central South University(Medical Sciences)
Year:
2006
Type:
Article