Determination of rhodamine 123 in cell lysate by high-performance liquid chromatography with visible wavelength detection / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 610-612, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-813637
ABSTRACT
OBJECTIVE@#To establish a high-performance liquid chromatography with visible wavelength detection method for rhodamine 123 in cell lysate.@*METHODS@#The HPLC separation was performed on a Kromasil C(18) (5 microm, 4.6 mm x 250 mm) column, using water (15 mmol/L potassium acetate and 1.2 mmol/L tetrabutylammonium bromide) - acetonitrile (6535) as the mobile phase. The wave length was 390 nm, the internal standard was rhodamine B. Protein in the sample was precipitated by trichloroacetic acid.@*RESULTS@#The calibration curve was linear in the range of 3 - 300 microg/L. The intra-day and inter-day RSDs were less than 5%.@*CONCLUSION@#The method is accurate,sensitive, simple, and reliable for determining rhodamine 123 in cell lysate.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Spectrophotometry
/
Reproducibility of Results
/
Chromatography, High Pressure Liquid
/
Rhodamine 123
/
Methods
Type of study:
Diagnostic study
Limits:
Animals
/
Humans
Language:
Chinese
Journal:
Journal of Central South University(Medical Sciences)
Year:
2006
Type:
Article
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