Primary culture of human omental preadipocytes / 中南大学学报(医学版)

ABSTRACT

OBJECTIVE@#To establish a primary culture method of human omental preadipocytes.@*METHODS@#Using enzyme-digesting method, fibroblast-like cells from the human omental adipose tissues were cultured, and then differentiated by conditional medium, and identified by oil red O staining.@*RESULTS@#The cultured cells were highly homogeneous, and highly proliferative in 4-5th generation. During the process of induction by conditional medium, the cells became round-like and larger, and more adipose droplets were aggregated. By oil red O staining, we confirmed the differentiated cells were mature adipocytes.@*CONCLUSION@#In human omental adipose tissues, there are some preadipocytes, which can differentiate into mature adipocytes with appropriate stimulus.