Identification and clone of human Alzheimer's disease related gene nicastrin promoter / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 9-13, 2006.
Article
in Chinese
| WPRIM
| ID: wpr-813777
ABSTRACT
OBJECTIVE@#To identify the promoter of human nicastrin (NCT) gene, a major component of gamma-secretase which is closely related with pathogenesis of Alzheimer's disease.@*METHODS@#Promoter of human Alzheimer's disease related gene, nicastrin, a 1768 bp fragment was firstly isolated from human genomic DNA by PCR. This fragment's 3 flanking end was 4 bp upstream to the start codon ATG (+1) of the gene. This fragment was used as template, a series of deleted fragments were amplified and constructed to the pGL3-Enhancer plasmid with the artificial designed linkers. The relative activity of their promoter in Hela cells was studied by dual-luciferase assay.@*RESULTS@#The 420 bp fragment showed the strongest activity, and the 237 bp fragment was the minimal fragment in length with activity.@*CONCLUSION@#The promoter of NCT is located at -432/-133 region upstream the translational start codon, while its basal promoter is between -359/-90 that drives the transcription of reporter gene in Hela cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Membrane Glycoproteins
/
HeLa Cells
/
Promoter Regions, Genetic
/
Cloning, Molecular
/
Genes, Reporter
/
Amyloid Precursor Protein Secretases
/
Alzheimer Disease
/
Genetics
Type of study:
Diagnostic study
Limits:
Humans
Language:
Chinese
Journal:
Journal of Central South University(Medical Sciences)
Year:
2006
Type:
Article
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