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Protective effect of thrombin precondition on astrocytes insulted by oxygen-glucose deprivation / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences) ; (12): 845-849, 2007.
Article in Chinese | WPRIM | ID: wpr-813788
ABSTRACT
OBJECTIVE@#To explore the effect of thrombin precondition (TPC) on the rat cerebral astrocytes(As) cultured in oxygen-glucose deprivation (OGD).@*METHODS@#Astrocytes were pretreated with thrombin (TB) at various concentrations (0.005 approximately 5.000 kU/L), and then insulted by OGD. The cell damage and viability were evaluated by the lactate dehydrogenase (LDH) effusion rate and the 3-(4,5-dimethylthazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) conversion method. Detection of apoptotic cells was determined by the flow cytometry technique. The glutamate uptake of astrocytes was studied with [3H]-glutamate incorporation.@*RESULTS@#OGD increased the LDH, decreased the cell viability, increased the number of apoptotic astrocytes, and decreased the glutamate uptake (P<0.01). While preconditioned with thrombin at the same condition, the LDH decreased, the cell viability increased, the percentage of apoptotic cells decreased, and the glutamate uptake increased (P<0.05). The maximum protective effect of thrombin was observed at 0.1 kU/L.@*CONCLUSION@#Low concentration of thrombin precondition (TPC) can protect the astrocytes from oxygen-glucose deprived injury, and attenuate its apoptosis in a dose-dependent manner.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Oxygen / Pharmacology / Thrombin / Cell Hypoxia / Cells, Cultured / Astrocytes / Rats, Sprague-Dawley / Apoptosis / Neuroprotective Agents / Glutamic Acid Limits: Animals Language: Chinese Journal: Journal of Central South University(Medical Sciences) Year: 2007 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Oxygen / Pharmacology / Thrombin / Cell Hypoxia / Cells, Cultured / Astrocytes / Rats, Sprague-Dawley / Apoptosis / Neuroprotective Agents / Glutamic Acid Limits: Animals Language: Chinese Journal: Journal of Central South University(Medical Sciences) Year: 2007 Type: Article