Effect of calcitonin-gene-related peptide on MMP-9 production in human bronchial epithelial cells / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 771-775, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-813801
ABSTRACT
OBJECTIVE@#To examine the expression of matrix metalloproteinase-9 (MMP-9) in human bronchial epithelial cells treated with calcitonin-gene-related peptide (CGRP).@*METHODS@#RT-PCR and gelatin zymography were performed to examine the dynamic expression and activity of MMP-9 in human bronchial epithelial cells at different doses (10(-10), 10(-9), 10(-8), 10(-7), and 10(-6)mol/L) and different time points (6,12,18,24,36, and 48h) after the stimulation of CGRP.@*RESULTS@#The unstimulated human bronchial epithelial cells only secreted a small amount of MMP-9. After the CGRP stimulation, the expression of MMP-9 presented in a concentration-dependent (10(-10), 10(-9), 10(-8), 10(-7), and 10(-6) mol/L) and time-dependent (6,12,18,24,36, and 48 h) manners (P<0.01) in human bronchial epithelial cells. The effect of CGRP could be diminished by H-7 and W-7, an antagonist of protein kinase C (PKC) and calmodulin (CaM) (P<0.05).@*CONCLUSION@#CGRP can stimulate the secretion and expression of MMP-9 in human bronchial epithelial cells, and the signal transduction is partly via the PKC and CaM pathway.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Protein Kinase C
/
Bronchi
/
Calmodulin
/
Signal Transduction
/
Calcitonin Gene-Related Peptide
/
Cells, Cultured
/
Matrix Metalloproteinase 9
/
Cell Biology
/
Epithelial Cells
Limits:
Humans
Language:
Chinese
Journal:
Journal of Central South University(Medical Sciences)
Year:
2007
Type:
Article
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