Intrasplenic tumor model of nude mice in the anti- metastasis roles of NGX6 gene against colon cancer / 中南大学学报(医学版)

ABSTRACT

OBJECTIVE@#To establish a liver metastasis model of nude mice in colon cancer so as to determine the function of NGX6.@*METHODS@#The cells of Group HT-29, pcDNA3.1(+)/HT-29, and pcDNA3.1(+)/NGX6/HT-29 were implanted into the spleen of nude mice, respectively. Everyday we measured the weight of the nude mice and observed their ingestion, movement and mental status. The nude mice were killed after 45 days, and the effect of NGX6 on the malignant behavior of HT-29 was assessed by this experiment.@*RESULTS@#In contrast to the other two groups, the metastasis in the liver and xenograft tumor in the spleen of pcDNA3.1(+)/NGX6/HT-29 group was significantly reduced (P<0.01).@*CONCLUSION@#The metastasis of HT-29 colon cancer cell line was significantly inhibited by NGX6 gene. This model of liver metastasis in the nude mice is a proper model to determine the anti-metastasis mechanism of NGX6 gene.