Effect of ulinastain on the expression of hemeoxygenase-1 in oleic acid-induced acute lung injury in rats / 中南大学学报(医学版)

ABSTRACT

OBJECTIVE@#To explore the effect of ulinastain on the expression of hemeoxy genase-1 (HO-1) in oil acid-induced acute lung injury in rats.@*METHODS@#The animal model of acute lung injury was established by oil acid. Thirty SD rats were randomly divided into 3 groups the blank control group (A), the acute lung injury group (B) and the acute lung injury group (C) followed by injecting 100 mL/kg ulinastatin. Each group consisted of 10 rats. Group A were given 0.2 mL/kg natural solution through the trial vein; Group B and C were given 0.2 mL/kg oil-acid through trial vein, while group C were injected 100mL/kg ulinastatin by the peritoneal cavity after injecting oil acid. After 4 hours, the rates of respiration were counted and blood samples were cramped out through the heart puncture for blood gas analysis. The expressions of hemeoxygenase-1 and the pathologic construction changes were determined by HE staining in the lower right lung of rats in the 3 groups.@*RESULTS@#The respiration dysfunction caused by oil acid could be prominently improved by ulinastain. There was only a little expression of hemeoxygenase-1 in the lung of Group A, but the expression increased in Group B and significatively increased in Group C.@*CONCLUSION@#Ulinastatin may protect the rats from acute lung injury through increasing the expression of HO-1.