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Antisense c-fos oligonucleotides-induced myopia in guinea pigs / 中南大学学报(医学版)
Zhongnan Daxue xuebao. Yixue ban ; (12): 132-137, 2007.
Article in Zh | WPRIM | ID: wpr-813922
Responsible library: WPRO
ABSTRACT
OBJECTIVE@#To characterize the antisense c-fos oligonucleotides that control the expression of immediate-early gene c-fos in retina in order to better understand the mechanism by which antisense c-fos oligonucleotides induced myopia. In this study the signal transduction in the pathway linking visual experience and the regulation of the eye's growth was investigated.@*METHODS@#Thirty-one 3-week guinea pigs were assigned into 3 groups: antisense and sense c-fos oligonucleotides were intravitreally injected every 3 days to the eyes of the experimental guinea pigs at different concentrations; and saline vehicle to control guinea pigs in the same way. The refraction and axial length of the eyes were measured before and after the treatment, and the immediate-early gene c-fos expression in the retina was quantified by immunohistochemistry and RT-PCR.@*RESULTS@#The moderate myopia was induced in high (1 nmol) and low (0.1 nmol) level of antisense c-fos oligonucleotide intravitreous injection (-5.425 D and -5.575 D, respectively) compared with the control ateral eyes. The refraction and axial length of the treated eyes increased, and the expression of immediate-early gene c-fos decreased significantly in the antisense c-fos oligonucleotides intravitreously injected eyes compared with the sense c-fos oligonucleotide intravitreously and saline vehicle injected eyes (P0.05).@*CONCLUSION@#The obvious myopia can be induced by antisense c-fos oligonucleotides in guinea pigs; antisense c-fos oligonucleotides inhibit c-fos expression in the retina. Immediate-early gene c-fos may be a potential factor in the prevention of myopia and plays an important role in the signal transduction of the retina.
Subject(s)
Full text: 1 Index: WPRIM Main subject: Physiology / Retina / RNA, Messenger / Immunohistochemistry / Signal Transduction / Random Allocation / Oligonucleotides, Antisense / Proto-Oncogene Proteins c-fos / Genes, Immediate-Early / Reverse Transcriptase Polymerase Chain Reaction Limits: Animals Language: Zh Journal: Zhongnan Daxue xuebao. Yixue ban Year: 2007 Type: Article
Full text: 1 Index: WPRIM Main subject: Physiology / Retina / RNA, Messenger / Immunohistochemistry / Signal Transduction / Random Allocation / Oligonucleotides, Antisense / Proto-Oncogene Proteins c-fos / Genes, Immediate-Early / Reverse Transcriptase Polymerase Chain Reaction Limits: Animals Language: Zh Journal: Zhongnan Daxue xuebao. Yixue ban Year: 2007 Type: Article