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Effect of Krüppel-like factor 4 overexpression on heat stress-induced apoptosis of Raw264.7 macrophages / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences) ; (12): 1002-1006, 2007.
Article in Chinese | WPRIM | ID: wpr-813960
ABSTRACT
OBJECTIVE@#To observe the effect of Krüppel-like factor 4 (KLF4) overexpression on heat stress-induced apoptosis of Raw264.7 macrophages.@*METHODS@#The fragment containing full length mouse KLF4 cDNA coding sequence was inserted into the pcDNA3.1 vector and Raw264.7 macrophages were transfected with pcDNA3.1-KLF4 plasmids using lipofectamine.The expression of KLF4 was examined by Western blot in the Raw264.7 macrophages stably transfected with pcDNA3.1- KLF4 plasmids. Raw264.7 cells transfected with pcDNA3.1 and pcDNA3.1-KLF4 were exposed to heat stress (42 degrees C) for 1h and recovered at 37 degrees C for 12h. Flow cytometry, Hoechest 33258 staining assay, and DNA ladder assays were performed to assess the apoptosis.@*RESULTS@#The KLF4 overexpressed Raw264.7 macrophages were established. After the heat stress, flow cytometry showed that apoptotic cells increased significantly in KLF4 overexpressed cells compared with the vector control; Hoechest 33258 staining was characterized with classical changes including apoptotic body, and nuclear condensation. Agarose gel electrophoresis showed that "DNA ladder" could be observed clearly.@*CONCLUSION@#KLF4 overexpression can increase heat stress-induced apoptosis of Raw264.7 macrophages.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Plasmids / Transfection / Cell Line / Apoptosis / Heat-Shock Response / Cell Biology / Kruppel-Like Transcription Factors / Genetic Vectors / Genetics / Macrophages Limits: Animals Language: Chinese Journal: Journal of Central South University(Medical Sciences) Year: 2007 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Plasmids / Transfection / Cell Line / Apoptosis / Heat-Shock Response / Cell Biology / Kruppel-Like Transcription Factors / Genetic Vectors / Genetics / Macrophages Limits: Animals Language: Chinese Journal: Journal of Central South University(Medical Sciences) Year: 2007 Type: Article