Differential proteomic analysis of nasopharyngeal carcinoma cell lines 5-8F and 6-10B / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 978-984, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-813964
ABSTRACT
OBJECTIVE@#To compare the proteome difference of nasopharyngeal carcinoma (NPC) cell lines 5-8F and 6-10B, and to screen these proteins associated with NPC metastasis.@*METHODS@#Two-dimensional gel electrophoresis (2-DE) was used to separate the total proteins from NPC cell lines 5-8F and 6-10B with different metastatic potentials and same genetic background, respectively. PDQuest software was applied to analyze 2-DE images, and the differentially expressed protein spots between 5-8F and 6-10B were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). The expression levels of partial identified proteins in the 2 cell lines were detected by Western blot.@*RESULTS@#2-DE maps of total proteins from 5-8F and 6-10B were established. A total of 65 differential protein spots in the 2 cell lines were found, and 15 non-redundant differential expression proteins were identified by MALDI-TOF-MS. Western blot showed that Annexin A1 and 14-3-3 protein sigma were differential expression proteins in 5-8F and 6-10B, which was consistent with the Results from the comparative proteomic analysis.@*CONCLUSION@#Fifteen non-redundant differential expression proteins are useful for studying the metastatic mechanism of NPC.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Mass Spectrometry
/
Carcinoma
/
Electrophoresis, Gel, Two-Dimensional
/
Nasopharyngeal Neoplasms
/
Proteome
/
Proteomics
/
Cell Line, Tumor
/
Nasopharyngeal Carcinoma
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Journal of Central South University(Medical Sciences)
Year:
2007
Type:
Article
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