Effect of sodium tungstate on glucose metabolism in adipocytes / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 727-730, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-814007
ABSTRACT
OBJECTIVE@#To explore the effect of sodium tungstate on glucose metabolism in adipocytes and its mechanism.@*METHODS@#After 3T3-L1 preadipocytes were differentiated into adipocytes, these adipocytes were incubated with sodium tungstate (0, 150, 300, 500, and 700 micromol/L) for 48 h, and then glucose consumption of the adipocytes was detected by glucose-oxidase assay. Glucose transport was determined by the uptake of 2-deoxy-[3H]-D-glucose, and the expression of glucose transport-4 (GLUT-4) mRNA was identified by semi-quantitative RT-PCR.@*RESULTS@#Sodium tungstate (150 approximately 700 micromol/L) could significantly increase the glucose consumption and glucose transport with a concentration dependent-effect. Sodium tungstate could increase GLUT-4 mRNA expression.@*CONCLUSION@#Sodium tungstate can enhance the glucose metabolism of adipocytes by up-regulating the expression of GLUT-4 mRNA.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
RNA, Messenger
/
Up-Regulation
/
Tungsten Compounds
/
Adipocytes
/
3T3-L1 Cells
/
Glucose Transporter Type 4
/
Genetics
/
Glucose
/
Hypoglycemic Agents
Limits:
Animals
Language:
Chinese
Journal:
Journal of Central South University(Medical Sciences)
Year:
2008
Type:
Article
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