Effect of FGF-2 on survivin and subcellular location of Smac in human small cell lung cancer cell NCI-H446 / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 705-711, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-814011
ABSTRACT
OBJECTIVE@#To investigate the effect of basic fibroblast growth factor (FGF-2)on survivin and subcellular location of Smac in human small cell lung cancer (SCLC) cell NCI-H446.@*METHODS@#Western blot was used to detect the expression of survivin protein induced by FGF-2. The release of Smac from mitochondria to cytoplasm affected by FGF-2 was observed by Western blot and immunofluorescence. Apoptosis of NCI-H446 cells was detected with flow cytometry and Hoechst 33258 staining.@*RESULTS@#The expression of survivin could be up-regulated in response to FGF-2 treatment in NCI-H446 cells, and the level of survivin expression is related to the concentration and time of FGF-2 treatment. FGF-2 could inhibit the release of Smac from the mitochondria to cytoplasm induced by serum starving. FGF-2 could inhibit the apoptosis induced by serum starving.@*CONCLUSION@#FGF-2 up-regulates the expression of survivin protein in NCI-H446 cells, and blocks the release of Smac from mitochondria cytoplasm. Survivin and Smac might play important roles in the apoptosis inhibited by FGF-2.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Tumor Cells, Cultured
/
Fibroblast Growth Factor 2
/
Apoptosis
/
Cytoplasm
/
Mitochondrial Proteins
/
Intracellular Signaling Peptides and Proteins
/
Apoptosis Regulatory Proteins
/
Inhibitor of Apoptosis Proteins
Limits:
Humans
Language:
Chinese
Journal:
Journal of Central South University(Medical Sciences)
Year:
2008
Type:
Article
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