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Expression and SNP analysis of BRD7 gene in acute leukemia cells / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences) ; (12): 645-650, 2008.
Article in Chinese | WPRIM | ID: wpr-814021
ABSTRACT
OBJECTIVE@#To evaluate the expression level of BRD7 gene in bone marrow mononuclear cells (BMNCs) in patients with acute leukemia (AL) and to analyze BRD7 single nucleotide polymorphism(SNP).@*METHODS@#RT-PCR was used to detect BRD7 expression in patients with AL and normal bone marrow subjects. Single-strand conformation polymorphism and DNA sequence analysis were also used to identify BRD7 mutation or SNP to investigate the relation between BRD7 and AL.@*RESULTS@#BRD7 mRNA in BMNCs from 52 patients with AL and 30 control subjects was expressed. The mRNA relative expression of BRD7 in patients with AL was higher than that of the control group (P=0.001). Three SNPs (C657A,C495T and A737G) in BRD7 gene coding region (447 approximately 844 bp) were found, and A737G was coupled with C495T . The allele frequencies of SNP C657A were not significantly different between AL and the control group. The genotype and the allele frequencies of the 2 coupled SNPs were significantly different (P0.05).@*CONCLUSION@#Expression of BRD7 gene is up-regulated in AL cells. The 2 coupled SNPs (C495T and A737G ) in BRD7 gene coding region (447 approximately 844 bp) are correlated with AL, indicating that SNPs may be one of the genetic susceptibility factors of AL.
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Full text: Available Index: WPRIM (Western Pacific) Main subject: RNA, Messenger / Molecular Sequence Data / Base Sequence / Chromosomal Proteins, Non-Histone / Leukemia, Myeloid, Acute / Amino Acid Sequence / Polymorphism, Single Nucleotide / Precursor Cell Lymphoblastic Leukemia-Lymphoma / Gene Frequency / Genetics Type of study: Prognostic study Limits: Adolescent / Adult / Aged / Child / Female / Humans / Male Language: Chinese Journal: Journal of Central South University(Medical Sciences) Year: 2008 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: RNA, Messenger / Molecular Sequence Data / Base Sequence / Chromosomal Proteins, Non-Histone / Leukemia, Myeloid, Acute / Amino Acid Sequence / Polymorphism, Single Nucleotide / Precursor Cell Lymphoblastic Leukemia-Lymphoma / Gene Frequency / Genetics Type of study: Prognostic study Limits: Adolescent / Adult / Aged / Child / Female / Humans / Male Language: Chinese Journal: Journal of Central South University(Medical Sciences) Year: 2008 Type: Article