Effect of pinggan qianyang on hypothalamic proteome in the hyperthyroid rats with hyperactivity of liver-yang / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 391-398, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-814066
ABSTRACT
OBJECTIVE@#To investigate the effect of pinggan-qianyang (PGQY), a Chinese medicine, on hypothalamic proteome in the hyperthyroid rats with hyperactivity of liver-yang, and to explore its mechanism.@*METHODS@#The rat model was established by intraperitoneal injection of levo-thyroxine (L-T4) and fuzi decotion. All the quantitative and qualitative changes of the protein expressions were compared among the normal group,the model group and the treatment group by proteomic techniques.@*RESULTS@#The protein spots in the 3 groups were mainly displayed at the isoelectric point (pI) 3 approximately 10, and the molecular weights were 13.8 approximately 98.8 kD.Compared with the normal group, 6 spots of protein expression increased and 10 decreased in the model group. All the changed protein in the model group returned to normal level after PGQY treatment. Mass-spectrometer and bio-informatics indicated that these proteins were Prohibitin, Peroxiredoxin-6, histidine triad nucleotide-binding protein 1, protein-tyrosine-phosphatase, predicted protein, profilin-2, peroxir doxin-II, heat shock protein-27, and annexin-A1.@*CONCLUSION@#There are differences in the expression of hypothalamus proteins in the hyperthyroid rats with hyperactivity of liver-yang after the treatment with PGQY, and the 9 identified protein spots may be associated with the mechanism of PGQY.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Repressor Proteins
/
Drugs, Chinese Herbal
/
Random Allocation
/
Rats, Sprague-Dawley
/
Proteome
/
Therapeutic Uses
/
Drug Therapy
/
Peroxiredoxin VI
/
Hyperthyroidism
/
Hypothalamus
Type of study:
Diagnostic study
/
Prognostic study
/
Qualitative research
Limits:
Animals
Language:
Chinese
Journal:
Journal of Central South University(Medical Sciences)
Year:
2008
Type:
Article
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