Construction of eukaryotic expression vector encoding human nasopharyngeal carcinoma anti-idiotype antibody single chain fragment gene G22 and its expression / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 16-20, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-814131
ABSTRACT
OBJECTIVE@#To construct a eukaryotic expression vector encoding human nasopharyngeal carcinoma anti-idiotype antibody single chain fragment (ScFv) gene G22, and to identify its expression in rectal cancer cells (CMT-93).@*METHODS@#The G22 gene was ligated into the sites of EcoRI and NotI of eukaryotic expression vector pcDNA3.1(+). After the identification and DNA sequencing, the recombinant plasmid pc DNA3.1(+)-G22 was stably transfected into CMT-93 cells, and the expression of G22 was detected by Western blot, flow cytometry and immunofluorescence staining.@*RESULTS@#Restriction enzyme digestion and DNA sequencing showed that the recombinant plasmid contained the human nasopharyngeal carcinoma anti-idiotype antibody ScFv gene G22. Transfection experiment verified that G22 gene could be expressed in CMT-93 cells in the right way.@*CONCLUSION@#The eukaryotic expression vector containing the human nasopharyngeal carcinoma anti-idiotype antibody ScFv gene G22 is successfully constructed and expressed, which is the basis for further study of its DNA vaccine.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Recombinant Proteins
/
Immunoglobulin Variable Region
/
Nasopharyngeal Neoplasms
/
Antibodies, Anti-Idiotypic
/
Allergy and Immunology
/
Eukaryotic Cells
/
Genetic Vectors
/
Genetics
/
Metabolism
/
Antibodies, Neoplasm
Type of study:
Prognostic study
Limits:
Humans
Language:
Chinese
Journal:
Journal of Central South University(Medical Sciences)
Year:
2008
Type:
Article
Similar
MEDLINE
...
LILACS
LIS