Expression of Class I integrase gene in Acinetobacter baumannii and drug-resistance / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 952-957, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-814169
ABSTRACT
OBJECTIVE@#To explore the expression level of class I integrase (intI 1) mRNA in Acinetobacter baumannii from biofilm cells and planktonic cultured cells ,and to analyze the drug-resistance of Class I integron positive strains.@*METHODS@#Acinetobacter baumannii were collected from hospitals,and Class I integron strains were screened by gene amplification. Total RNA of Class I integron positive strains was extracted, and the intI1 mRNA expression in the bioflim cells and planktonic cultured cells was measured by RT-PCR. Susceptibilities to antibiotics of Class I integron positive strains were also examined.@*RESULTS@#The intI1 gene mRNA was expressed under 2 conditions, and the mRNA expressed in the biofilm cells was about 4 times higher than that in the planktonic cultured cells. Among the 64 strains of Acinetobacter baumannii, 46 strains were Class I integron positive strains. The antibiotic resistance of intI1 gene cassette-positive strains was higher than that of gene cassette-negative strains.@*CONCLUSION@#The intI1 gene mRNA can be up-regulated in Acinetobacter baumannii biofilm cells.Class I integron plays an important role in drug resistance. It is much easier to capture gene cassettes for bacteria under biofilm condition.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Acinetobacter Infections
/
RNA, Messenger
/
Molecular Sequence Data
/
Base Sequence
/
Drug Resistance, Multiple
/
Biofilms
/
Integrases
/
Acinetobacter baumannii
/
Genetics
/
Microbiology
Limits:
Humans
Language:
Chinese
Journal:
Journal of Central South University(Medical Sciences)
Year:
2008
Type:
Article
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