Expression of plasma thrombospondin-1 in renal interstitial fibrosis / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 796-802, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-814269
ABSTRACT
OBJECTIVE@#To observe the expression of plasma thrombospondin-1(TSP-1) at different time in protein-overload rats and to analyze the relationship between plasma TSP-1 expression and renal interstitial fibrosis.@*METHODS@#Forty-five male Sprague-Dawley rats were randomly divided into a bovine serum albumin (BSA) group and a control group after uninephrectomization. Rats with protein overload nephropathy induced by intraperitoneally injected BSA were used as a model (control group received saline). At the 1st, 5th, and 9th weekend, the level of 24 h proteinuria and renal function was assessed. Pathological changes were observed by electron and fluorescent microscopy. The expression of plasma TSP-1 was detected by Western blot. The relationship between plasma TSP-1 and tubulointerstitial lesions (TIL) score was analyzed.@*RESULTS@#Twenty-four hour proteinuria and blood urea nitrogen (BUN) significantly increased in protein-overload rats compared with those in the control group. While protein-overload rats developed more severe fibrosis in the tubular and interstitium. Glomerulosclerosis index and TIL score were upregulated compared with those in the control group. The expression of TSP-1 increased significantly at the 5th and 9th weekend. The expression of TSP-1 was positively correlated with TIL score (r=0.836, P<0.01).@*CONCLUSION@#Plasma TSP-1 expression is positively correlated with renal interstitial fibrosis in protein-overload rats. Plasma TSP-1 may be used for an important biomarker of renal interstitial fibrosis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Blood
/
Fibrosis
/
Glomerulosclerosis, Focal Segmental
/
Rats, Sprague-Dawley
/
Thrombospondin 1
/
Kidney
/
Metabolism
/
Nephrectomy
/
Nephritis, Interstitial
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Journal of Central South University(Medical Sciences)
Year:
2009
Type:
Article
Similar
MEDLINE
...
LILACS
LIS