Effect of CpG motif containing oligodeoxynucleotides on dendritic cell function in patients with chronic hepatitis B / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 461-467, 2009.
Article
in English
| WPRIM
| ID: wpr-814303
ABSTRACT
OBJECTIVE@#To explore the effect of unmethylated CpG motif containing oligodeoxynucleotides (CpG ODN) on the function of dendritic cells (DCs) in patients with chronic hepatitis B (CHB).@*METHODS@#DCs were obtained from peripheral blood mononuclear cells (PBMCs) of 15 CHB patients, 12 hepatitis B virus (HBV) carriers, and 10 healthy controls. The expressions of HLA-DR, CD80, and CD86 on DCs were determined by fluorescence activated cell sorting (FACS). The IL-12 level in supernatant of the culture medium was measured by ELISA, and the morphological changes of DCs were observed under transmission electron microscope.@*RESULTS@#Compared with the controls, DCs stimulated with CpG ODN represented enrichment in cell surface protrusions and rough endoplasmic reticulum, decreased or disappeared vacuole. The expressions of HLA-DR, CD86, and CD80 were much higher in DCs stimulated with CpG ODN than those in complete medium control (P<0.05). When culturing in complete medium, the expressions of HLA-DR, CD86, and CD80 were much lower in CHB patients and HBV carriers than healthy controls (P<0.05). The expressions of HLA-DR and CD86 stimulated with CpG ODN were much lower in CHB patients than HBV carriers and healthy controls (P<0.05). The expressions of CD80 were much lower in CHB patients and HBV carriers than healthy controls (P<0.05). The levels of IL-12 were much higher in DCs stimulated with CpG ODN than that in complete medium controls (P<0.05). The levels of IL-12 in complete medium or medium added with CpG ODN were much lower in CHB patients and HBV carriers than in healthy controls (P<0.05).@*CONCLUSION@#CpG ODN could significantly promote the maturation of dendritic cells in peripheral blood in CHB patients.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oligodeoxyribonucleotides
/
Pharmacology
/
Dendritic Cells
/
Carrier State
/
HLA-DR Antigens
/
Case-Control Studies
/
B7-1 Antigen
/
CpG Islands
/
Hepatitis B, Chronic
/
Allergy and Immunology
Type of study:
Observational study
Limits:
Adult
/
Female
/
Humans
/
Male
Language:
English
Journal:
Journal of Central South University(Medical Sciences)
Year:
2009
Type:
Article
Similar
MEDLINE
...
LILACS
LIS