Effect of High Glucose on the Oxidative Stress in Trabecular Meshwork Cells
Journal of the Korean Ophthalmological Society
;
: 1563-1568, 2009.
Article
in Korean
| WPRIM
| ID: wpr-81433
ABSTRACT
PURPOSE:
To investigate the effect of high glucose (HG) on the oxidative stress in cultured human trabecular meshwork cells (HTMC).METHODS:
Primarily cultured HTMC were exposed to low glucose (5 mM) and HG (25 mM) for 7 days. Additionally, 1 mM L-arginine, 5 mM DAHP, 10 microgram/ml insulin, 100 micrometer L-ascorbic acid, 10, and 100 micrometer sepiapterin were co-exposed. The cellular survival and nitric oxide (NO) production were assessed by MTT assay and Griess assay, respectively. Superoxide production was measured by modified cytochrome c assay.RESULTS:
HG did not affect the survival of cultured HTMC significantly. HG decreased NO production. Co-exposed DAHP decreased but DAHP and insulin increased NO production. In addition, HG increased superoxide production, which was decreased by insulin, L-ascorbic acid, and sepiapterin.CONCLUSIONS:
HG decreased NO production accompanied with increased superoxide production in HTMC. Thus HG induces oxidative stress in HTMC and may cause cellular dysfunction and damage of the trabecular meshwork.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Arginine
/
Pterins
/
Ascorbic Acid
/
Sugar Acids
/
Trabecular Meshwork
/
Superoxides
/
Oxidative Stress
/
Cytochromes c
/
Glucose
/
Insulin
Limits:
Humans
Language:
Korean
Journal:
Journal of the Korean Ophthalmological Society
Year:
2009
Type:
Article
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