SARA expression in high glucose induced epithelium to mesenchymal transition of proximal tubule cells / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 1230-1235, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-814340
ABSTRACT
OBJECTIVE@#To construct the cell model of epithelium to mesenchymal transition of proximal tubule cells induced by high glucose and to determine the expression of Smad anchor for receptor activation (SARA).@*METHODS@#Protein expression of vimentin, Zona occludens-1(ZO-1), and SARA was determined by Western blot, and their mRNA expressions were detected by Real-time PCR.@*RESULTS@#After stimulation by 30 mmol/L D-glucose, the protein and mRNA expression levels of vimentin in HK-2 cells increased in a time-dependent manner while the expression of ZO-1 was reduced significantly, especially at 48 h. Meanwhile, SARA was also decreased in a time-dependent manner.@*CONCLUSION@#High glucose can induce renal epithelium to mesenchymal transition, and SARA may be involved in this process as a protector.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Phosphoproteins
/
Vimentin
/
RNA, Messenger
/
Serine Endopeptidases
/
Signal Transduction
/
Cell Differentiation
/
Cells, Cultured
/
Cell Biology
/
Intracellular Signaling Peptides and Proteins
Type of study:
Prognostic study
Limits:
Humans
Language:
Chinese
Journal:
Journal of Central South University(Medical Sciences)
Year:
2010
Type:
Article
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