Effect of celecoxib and adriamycin on proliferation and apoptosis of Raji cells and its mechanism / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 1085-1090, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-814356
ABSTRACT
OBJECTIVE@#To assess the effect of celecoxib in combination with adriamycin(ADM) on cell proliferation and apoptosis.@*METHODS@#Cell lines were treated with celecoxib,and cell proliferation was examined by the method of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) at different time. The cellular apoptotic rate was analyzed by flow cytometry. RT-PCR was applied to distinguish the level of caspase-9 mRNA expression. Immunohistochemistry was used to detect the caspase-9 protein expression in Raji cells.@*RESULTS@#Celecoxib inhibited the proliferation and increased the apoptosis of Raji cell lines in a dose-dependent and time-dependent manner within 20-100 μmol/L. Compared with the celecoxib alone, the proliferation of Raji cell lines incubated with celecoxib and adriamycin was decreased. Caspase-9 mRNA and protein expression in Raji cells were significantly enhanced after the treatment of celecoxib (P<0.05).@*CONCLUSION@#Celecoxib can inhibit the proliferation of Raji cells in a dose-dependent and time-dependent manner. Celecoxib may lead to the apoptosis of Raji cells by up-regulating activities of caspase-9. Adriamycin could intensify the effect.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Pyrazoles
/
Sulfonamides
/
RNA, Messenger
/
Doxorubicin
/
Burkitt Lymphoma
/
Apoptosis
/
Cell Line, Tumor
/
Cell Proliferation
Limits:
Humans
Language:
Chinese
Journal:
Journal of Central South University(Medical Sciences)
Year:
2010
Type:
Article
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