Cardioprotection of adenosine treatment in cadaver donor rats with warm ischemia / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 885-891, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-814378
ABSTRACT
OBJECTIVE@#To determine the cardioprotection of adenosine treatment in cadaver donor rats with warm ischemia.@*METHODS@#Rats were randomly divided into 7 groups. Group C was a control group. Group I(5), I(15), and I(30) The donor hearts from the cadaver rats that died of acute hemorrhagic shock and within warm ischemic durations of 5, 15, and 30 min, respectively, and the donor hearts were perfused with STH-1 for 30 min before cervical heterotopic heart transplantation. Group A(5), A(15), and A(30) were adenosine treatment groups and the donor hearts were obtained as mentioned above and perfused with STH-1 plus adenosine (adenosine concentration 1.2 mmol/L) before cervical heterotopic heart transplantation. We detected the change of the grafts including ultrastructure, the level of ATP, SOD, and MDA, NF-kappaB mRNA, TNF-alpha, and IL-6 24 h after the transplantation.@*RESULTS@#At the same time point, compared with Group I(5), I(15), and I(30), Group A(5), A(15), and A(30) showed increased ATP and SOD in the myocardial tissues, and decreased MDA, NF-kappaB mRNA, TNF-alpha, and IL-6 (P<0.05).@*CONCLUSION@#Adenosine treatment can protect the graft in cadaver donor rats with warm ischemia. The mechanism may improve the myocardial energy metabolism, attenuate lipid peroxidation injury, suppress the expression of NF-kappaB mRNA, and decrease the inflammatory damage of TNF-alpha and IL-6.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Organ Preservation
/
Pharmacology
/
Cardiotonic Agents
/
Myocardial Reperfusion Injury
/
Random Allocation
/
Adenosine
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Heart Transplantation
/
Rats, Sprague-Dawley
/
Warm Ischemia
/
Heart
Limits:
Animals
Language:
Chinese
Journal:
Journal of Central South University(Medical Sciences)
Year:
2010
Type:
Article
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