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Effect of GM6001 on the model that cervical squamous carcinoma cell line HCE1 multicellular spheroids invade live human umbilical vein endothelium cell monolayers / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences) ; (12): 868-874, 2010.
Article in Chinese | WPRIM | ID: wpr-814381
ABSTRACT
OBJECTIVE@#To investigate the relationship between matrix matrix metalloproteinases (MMP)-9 expression in HCE1/multicellular spheroids (MCS) and invasion of cervical carcinoma, and to explore the effect of MMPs inhibitor GM6001 on the model that HCE1/MCS invade live human umbilical vein endothelium cell (HUVEC).@*METHODS@#We established the invasion model by coculturing HCE1/MCS and HUVEC, and detected the MMP-9 expression in HCE1 monolayer cells (HCE1/MC), HCE1/MCS and HUVEC using immunohistochemistry and treated the HCE1/MCS invasion model with GM6001.@*RESULTS@#A cervical squamous carcinoma cell HCE1 infiltrating model was established. Compared with that in HCE1/MC, MMP-9 expression in the HCE1/MCS was significantly higher (P<0.05). Compared with the control group, the invasion of HCE1/MCS was inhibited by 26.09% and 92.95% (P<0.05) in the GM6001 2.5 μmol/L and 12.5 μmol/L group. MMP-9 expression in HCE1/MCS in the GM6001 12.5 μmol/L group was obviously lower than that in the control group (P<0.05). With the increase of the concentration of GM6001, the inhibition increased.@*CONCLUSION@#The enhancement of HCE1/MCS invasion may be related to the up-regulation of MMP-9 expression in HCE1/MCS. GM6001 can down regulate the MMP-9 expression in HCE1/MCS and partially block the HCE1/MCS invasion to HUVEC.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Pharmacology / Umbilical Veins / Carcinoma, Squamous Cell / Cell Line / Uterine Cervical Neoplasms / Spheroids, Cellular / Matrix Metalloproteinase 9 / Cell Biology / Cell Line, Tumor Limits: Female / Humans Language: Chinese Journal: Journal of Central South University(Medical Sciences) Year: 2010 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Pharmacology / Umbilical Veins / Carcinoma, Squamous Cell / Cell Line / Uterine Cervical Neoplasms / Spheroids, Cellular / Matrix Metalloproteinase 9 / Cell Biology / Cell Line, Tumor Limits: Female / Humans Language: Chinese Journal: Journal of Central South University(Medical Sciences) Year: 2010 Type: Article