Differential proteomic analysis of drug resistant A549/DDP cell lines in human lung adenocarcinoma / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 854-860, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-814384
ABSTRACT
OBJECTIVE@#To establish 2-dimensional electrophoresis (2-DE) graph of A549 and A549/DDP cell lines, to identify the differentially expressed proteins, and to screen multidrug resistance (MDR) related proteins in human lung adenocarcinoma.@*METHODS@#The total proteins of A549 and A549/DDP cells were obtained, and were extracted and separated by 2-DE. PDQuest software was applied to analyze the 2-DE images, and the differential proteins of the 2 types of cells were identified by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS). Western blot was used to determine the expression levels of the 4 proteins.@*RESULTS@#We established 2-DE maps of total proteins from A549 and A549/DDP. A total of 40 differential protein spots in the 2 cell lines were found, and 23 differential expression proteins were identified by MALDI-TOF-MS. Western blot showed that heat shock protein beta-1, annexin A4, cofilin l, vimentin were differential expression proteins in A549 and A549/DDP, which was consistent with the results of the comparative proteomic analysis.@*CONCLUSION@#The 23 differential expression proteins in human lung adenocarcinoma are useful for studying the MDR mechanism of lung adenocarcinoma.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Electrophoresis, Gel, Two-Dimensional
/
Adenocarcinoma
/
Classification
/
Annexin A4
/
Drug Resistance, Multiple
/
Drug Resistance, Neoplasm
/
Proteome
/
Proteomics
Limits:
Humans
Language:
Chinese
Journal:
Journal of Central South University(Medical Sciences)
Year:
2010
Type:
Article
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