Proteomic analysis of morphine rabbit myocardium with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 607-611, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-814411
ABSTRACT
OBJECTIVE@#To analyze the morphine rabbit myocardium with matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry (MALDI-TOF-MS).@*METHODS@#Six New Zealand white rabbits were randomly assigned to a control group (Group C) and a morphine group (Group M). Group C were pretreated with bolus injection of saline 1 mL/kg. Group M were pretreated with bolus injection of morphine 3 mg/kg. The myocardium tissue proteins of the rabbits 24 hours after the injection of morphine or saline preconditioned were extracted and separated by two dimensional gel electrophoresis(2-DE), and the images were analyzed and different proteins were found. Some of the different proteins were determined with MALDI-TOF-MS.@*RESULTS@#There were 51 protein spots that displayed quantitative changes in expression (P < 0.05), 15 protein spots were chosen for MS analysis, and 8 proteins were preliminarily identified.They were aldose reductase, zinc finger protein 312, src related tyrosine kinase, carbonic anhydrase 12 precursor, electron transfer flavoprotein beta-subunit, glyceraldehyde-3-phosphate dehydrogenase, tumor necrosis factor ligand superfamily member 11 and transmembrane emp24 domain-containing protein.@*CONCLUSION@#These proteins may be involved in the cardioprotection of morphine preconditioning.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Electrophoresis, Gel, Two-Dimensional
/
Myocardial Reperfusion Injury
/
Random Allocation
/
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
/
Ischemic Preconditioning, Myocardial
/
Proteome
/
Proteomics
/
Metabolism
/
Methods
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Journal of Central South University(Medical Sciences)
Year:
2010
Type:
Article
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