Effect of HMGB1 on human hepatoma cell line-HepG2 proliferation / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 451-457, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-814430
ABSTRACT
OBJECTIVE@#To investigate the effect of high mobility group box-1 protein (HMGB1) on the proliferative activity of human hepatoma cell line HepG2 and its potential regulating mechanism.@*METHODS@#The cultured HepG2 cells were treated with recombinant HMGB1 (0, 10, 50, and 100 ng/mL, respectively) for 24 h. Cell proliferation was observed by MTT analysis. Western blot and reverse transcriptase-polymerase chain reaction were used to detect the expression of proliferating cell nuclear antigen (PCNA) and cyclin D1 protein and mRNA, respectively.@*RESULTS@#Compared with the control group, HMGB1 at 10, 50, and 100 ng/mL obviously increased HepG2 cells proliferation, cyclin D1 and PCNA protein and mRNA expression after the treatment for 24 h, respectively (P<0.05). Anti-HMGB1 significantly inhibited the proliferation and cyclin D1 and PCNA mRNA and protein expression of HMGB1 on HepG2 cells (P<0.05).@*CONCLUSION@#Proliferation of HMGB1 on HepG2 cells may be associated with increasing cyclin D1 and PCNA expression. Anti-HMGB1 may have a therapeutic effect on hepatocellular carcinoma.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Recombinant Proteins
/
RNA, Messenger
/
Proliferating Cell Nuclear Antigen
/
Cyclin D1
/
Reverse Transcriptase Polymerase Chain Reaction
/
HMGB1 Protein
/
Cell Proliferation
/
Hep G2 Cells
/
Genetics
Limits:
Humans
Language:
Chinese
Journal:
Journal of Central South University(Medical Sciences)
Year:
2010
Type:
Article
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