Effect of neferine combined with mdr-1shRNA on the expression of mdr-1/P-gp in K562/A02 cell line / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 445-450, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-814431
ABSTRACT
OBJECTIVE@#To determine the effect of neferine (Nef) combined with mdr-1shRNA on the expression of mdr/P-gp in K562/A02 cell line.@*METHODS@#MTT assay was used to observe the cell proliferation. The expression level of P-gp was determined by Western blot and the transcription of mdr-1 gene was detected by semi-quantitative RT-PCR.@*RESULTS@#After K562/A02 cells were treated by Nef or mdr-1shRNA alone or both for 24 h, the proliferation of K562/A02 cells was significantly higher in the Nef combined with mdr-1shRNA treatment group than that of Nef or mdr-1shRNA alone group (P<0.01).The expression of mdr-1/P-gp in the Nef with mdr-1 shRNA group was significantly lower than that of Nef or mdr-1shRNA alone group.@*CONCLUSION@#Nef enhances the inhibition of mdr-1shRNA expression vector on K562/A02 cell proliferation and on P-gp protein to effectively reverse multidrug resistance induced by mdr-1 gene encoding P-gp.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Drugs, Chinese Herbal
/
ATP Binding Cassette Transporter, Subfamily B, Member 1
/
Drug Resistance, Multiple
/
Drug Resistance, Neoplasm
/
ATP Binding Cassette Transporter, Subfamily B
/
K562 Cells
/
RNA, Small Interfering
/
Benzylisoquinolines
/
Drug Synergism
Limits:
Humans
Language:
Chinese
Journal:
Journal of Central South University(Medical Sciences)
Year:
2010
Type:
Article
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