Effect of formaldehyde on expressions of Fas apoptosis pathway-related genes of ovary tissues in female rats / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 341-345, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-814444
ABSTRACT
OBJECTIVE@#To explore the mechanism of formaldehyde inducing ovarian toxicity in female rats by observing the effect of formaldehyde on the expression of Fas and caspase-8 mRNA, and the activity of caspase-3 and caspase-8 of ovary tissues in female rats.@*METHODS@#Forty female Sprague-Dawley(SD) rats were randomly divided into a control group and 3 formaldehyde groups at different concentrations. The rats in the formaldehyde groups were intraperitoneally injected different doses of formaldehyde (20.0,2.0 and 0.2 mg/kg) continuously for 14 days.After 14 days, all rats were sacrificed and their ovaries were collected for detecting the expression of Fas and caspase-8 mRNA with RT-PCR, the protein expression of Fas with Western blot, and the activities of caspase-8 and caspase-3 with spectrophotometric method.@*RESULTS@#Compared with the control group, the expression of Fas mRNA and its protein and caspase-8 mRNA and the activity of caspase-8 and caspase-3 of ovary tissues in the rats treated with formaldehyde significantly increased with dose (P<0.05).@*CONCLUSION@#The increase of Fas gene expression and the activity of caspase-8 and caspase-3 may be the important mechanism of ovarian toxicity induced by formaldehyde in female rats.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Ovary
/
Pathology
/
RNA, Messenger
/
Random Allocation
/
Rats, Sprague-Dawley
/
Apoptosis
/
Fas Receptor
/
Environmental Pollutants
/
Toxicity
/
Caspase 3
Limits:
Animals
Language:
Chinese
Journal:
Journal of Central South University(Medical Sciences)
Year:
2010
Type:
Article
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