Role of LRRN3 in the cerebellum postnatal development in rats / 中南大学学报(医学版)

ABSTRACT

OBJECTIVE@#To explore the effect and possible mechanism of LRRN3 in the cerebellum postnatal development in rats.@*METHODS@#New born rats were randomly divided into an experimental group and a control group, and each group included 3 sub-groups of different time points. Behavioral experiment, hematoxylin-eosin (HE) staining and immunohistochemistry were used to evaluate the effects of anti-LRRN3 injection on the cerebellum development in new born rats.@*RESULTS@#Compared with the control, the balance ability in the experiment group was weak, and there was significant difference in the static balance between the 2 groups (P<0.05). HE staining showed that molecular layer (ML) grew thicker from the 7th day to the 21st day after birth,and the structure changed dynamically. Vesicular glutamate transporter 1(VGluT1) expression was positive in the cerebellum of all groups, and the positive ML grew thicker from the 7th day to the 21st day after birth. Compared with the control, there was no obvious difference between the 2 groups on the 7th day after birth (P<0.05), while on the 14th day and the 21st day, there was significant difference (P<0.01).@*CONCLUSION@#LRRN3 plays an important role in cerebellum postnatal development. Anti-LRRN3 antibody injection may down-regulate the expression of VGluT1, reduce the synapse formation in the molecular layer,decrease the thickness of ML and inhibit the growth of cerebellum cortex and the functional neural circuit formation.