MYETS1 recombinant expression in prokaryotic cells and deletion analysis in multiple myeloma cell lines / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 27-31, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-814606
ABSTRACT
OBJECTIVE@#To explore the down-expression mechanism of MYETS1 gene in multiple myeloma cell lines ARH-77 or KM3, and express MYETS1 gene in prokaryotic express system.@*METHODS@#The region of chromosome 13q14.3 in ARH-77 and KM3 was detected by FISH. MYETS1 gene was amplified by RT-PCR and cloned into prokaryotic expression vector pGEX-4T.@*RESULTS@#Positive consequence was acquired in 13q14.3 where MYETS1 located by FISH in ARH- 77 and KM3 cell lines. Bioinformatics indicated highly sequence homology between MYETS1 and LECT1, but excluded the homology of open reading frame between MYETS1 and that of LECT1 by RT-PCR. Myets1 protein was expressed and harvested successfully.@*CONCLUSION@#The region of chromosome 13q14.3 ,where MYETS1 gene located, was not defected in ARH-77 and KM3 cell lines. Down-expression of MYETS1 might be regulated by other mechanisms in multiple myeloma cell lines.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Chromosomes, Human, Pair 13
/
Recombinant Proteins
/
Gene Deletion
/
Intercellular Signaling Peptides and Proteins
/
Cell Line, Tumor
/
Genetic Vectors
/
Genetics
/
Membrane Proteins
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Journal of Central South University(Medical Sciences)
Year:
2012
Type:
Article
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