Analysis of the relationship between the MecA gene and resistance of β-lactam antibiotics / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 567-571, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-814623
ABSTRACT
OBJECTIVE@#To investigate the mechanisms by which MecA gene expression leads to β-lactam resistance in methicillin-resistant Staphylococcus aureus (MRSA), and to study the resistance mechanism of MRSA at the molecular level.@*METHODS@#A variety of molecular biological techniques were employed, including screening MRSA using cefoxitin paper disk method, extraction of MRSA mRNA, reverse transcription into cDNA, real-time fluorescence PCR for quantitation of MecA gene expression, and agar dilution method for assessment of minimum inhibitory concentrations in MRSA treated with cefoxitin, oxacillin, vancomycin, or linezolid.@*RESULTS@#According to the level of resistance of MRSA to cefoxitin, 40 MRSA strains were divided into a low resistance group (n=12), a middle resistance group (n=15), and a high resistance group (n=13). The expression level of the MecA gene in the low resistance group, the middle resistance group, and the high resistance group was 58.87±30.30, 363.37±200.05, and 1257.72±446.63, respectively. MRSA resistance to cefoxitin and oxacillin was 100%; MRSA resistance to vancomycin or linezolid could not be detected. For all 40 MRSA strains the MIC90 for vancomycin was 2.0 μg/mL.@*CONCLUSION@#MecA gene expression levels may correlate with the MRSA level of resistance to cefoxitin within a certain range of concentration.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oxacillin
/
Pharmacology
/
Bacterial Proteins
/
Microbial Sensitivity Tests
/
Cefoxitin
/
Drug Resistance, Multiple, Bacterial
/
Penicillin-Binding Proteins
/
Methicillin-Resistant Staphylococcus aureus
/
Genetics
/
Metabolism
Language:
Chinese
Journal:
Journal of Central South University(Medical Sciences)
Year:
2012
Type:
Article
Similar
MEDLINE
...
LILACS
LIS