Effect of ultraviolet radiation on ALDH1 expression in human lens epithelial cells / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 572-577, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-814630
ABSTRACT
OBJECTIVE@#To determine the apoptosis-inducing effect of ultraviolet light (UV) on human lens epithelial cell (HLEC) and to explore the involvement of changes in ALDH1 folowing UV radiation.@*METHODS@#HLEC was exposed to the same UV light source and was subsequently divided into 6 groups according to UV radiation time of 0 (control group), 5, 10, 15, and 30 min. Apoptosis was detected by AO/EB staining. Changes of ALDH1 in HLEC were detected by immunohistochemical staining and Western blot.@*RESULTS@#The intensity of immunohistochemical staining and the rate of positive cells decreased with increase of UV time (P<0.05). The rate of positive ALDH1 cells was negatively correlated with the rate of apoptosis (r= -0.92, P<0.05). Western blot showed the integrated absorbance values significantly decreased with the increase of UV time (P<0.05).@*CONCLUSION@#ALDH1 in HLEC decreases with an increase of UV exposure, which may be related to UV induced apoptosis of HLEC.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Radiation Effects
/
Ultraviolet Rays
/
Cells, Cultured
/
Apoptosis
/
Cell Biology
/
Epithelial Cells
/
Retinal Dehydrogenase
/
Aldehyde Dehydrogenase 1 Family
/
Genetics
/
Isoenzymes
Limits:
Humans
Language:
Chinese
Journal:
Journal of Central South University(Medical Sciences)
Year:
2012
Type:
Article
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