Effect of calcitonin gene-related peptide on the maturation of oocyte in vitro / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 973-978, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-814738
ABSTRACT
OBJECTIVE@#To explore the effect of calcitonin gene-related peptide (CGRP) on murine oocyte maturation.@*METHODS@#After injection of pregnant mare serum gonadotropin (PMSG, 10 U, i.p.) for 48 h, 6-week old female Kunming mice were killed, and the cumulus oocyte complexes (COCs) were collected from ovaries and inoculated in the culture plate by 30-40/hole. The COCs were treated with 4 concentrations of CGRP (0, 10(-10), 10(-9), and 10(-8) mol/L), and the germinal vesicle breakdown (GVBD) and polar body I (PBI) were examined. Human granulosa cells were also cultured with CGRP (0, 10(-10), 10(-9), 10(-8) mol/L) and levels of intracellular cyclic adenosine monophosphate (cAMP) were measured.@*RESULTS@#Exogenous CGRP caused a decrease in GVBD and PBI in COCs, and an increase in cAMP levels in human granulosa cells in a concentration-dependent manner.@*CONCLUSION@#CGRP can inhibit the oocyte maturation, which may be related to the increased content of cAMP in granulosa cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oocytes
/
Pharmacology
/
In Vitro Techniques
/
Calcitonin Gene-Related Peptide
/
Cyclic AMP
/
Cell Biology
/
Granulosa Cells
/
Metabolism
Limits:
Animals
/
Female
/
Humans
Language:
Chinese
Journal:
Journal of Central South University(Medical Sciences)
Year:
2012
Type:
Article
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