hMLH1 gene promoter methylation in joint cartilage in patients with osteoarthritis / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 928-933, 2012.
Article
in Chinese
| WPRIM
| ID: wpr-814762
ABSTRACT
OBJECTIVE@#To investigate the role of Human MutL homologue 1 (hMLH1) gene promoter methylation in the occurrence and development of osteoarthritis (OA).@*METHODS@#General DNA was dealt with sodium bisulfite. The methylation of hMLH1 promoter was detected by methylation-specific PCR (MSP). hMLH1 protein expression in joint cartilage was detected by immunohistochemical method.@*RESULTS@#The positive percent of hMLH1 promoter methylation in OA patients was higher than that in healthy persons (χ(2)=30.634, P<0.001); the positive percent of hMLH1 protein in OA patients was significantly lower than that in healthy persons (χ(2)=37.724, P<0.001); prmoter methylation and protein expression level of hMLH1 gene showed negative correlation (rs=-0.554, P<0.001).@*CONCLUSION@#hMLH1 promoter is hypermethlated in joint cartilage cells of OA patients. Hypermethylation may affect the protein expression of hMLH1, which might play a role in the occurrence and development of OA.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Osteoarthritis
/
Nuclear Proteins
/
Cartilage, Articular
/
Promoter Regions, Genetic
/
DNA Methylation
/
Adaptor Proteins, Signal Transducing
/
MutL Protein Homolog 1
/
Genetics
/
Metabolism
Limits:
Adolescent
/
Adult
/
Child
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Journal of Central South University(Medical Sciences)
Year:
2012
Type:
Article
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