Identification of pathogenic microorganism by sequencing 16S rRNA gene / 中南大学学报(医学版)

ABSTRACT

OBJECTIVE@#To identify 14 bacteria by sequencing the 16S rRNA gene and establish the basis for clinical application in the future.@*METHODS@#DNA samples of the 14 bacteria were extracted. The 16S rRNA genes were amplified by PCR and sequenced with common primers. The sequences of the 16S rRNA genes were aligned by online software Blastn in nucleotide database. The bacteria were identified according to the homology of their 16S rRNA genes.@*RESULTS@#Twelve bacteria were classified to species, the other 2 bacteria were classified to genus.@*CONCLUSION@#16S rRNA gene sequence analysis is useful in identifying pathogenic bacteria.