Influence of BRAF interference on SW579 cell lines in thyroid cancer / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 576-581, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-814840
ABSTRACT
OBJECTIVE@#To determine the influence of v-raf murine sarcoma viral oncogene homolog B1 (BRAF) interference on SW579 cell lines in thyroid cancer.@*METHODS@#We designed 2 pairs of siRNA interference sequences, transfected them into SW579 cell line with liposome, and detected the interference with RT-PCR method. For successfully interfered cell lines, the changes in cell proliferation, cell cycle, and the expression of related proteins in mitogen-activated protein signal-regulated kinase /extracellular signal-regulated kinase (MEK/ ERK) signal pathway were detected.@*RESULTS@#After 2 pairs of siRNA transfection, the expressions of BRAF mRNA and protein of SW579 cell lines were significantly inhibited (P<0.01). The proliferation was inhibited, the cell cycle was changed, G1/S phase increased, and MEK/ERK signal pathway was inhibited.@*CONCLUSION@#Inhibition of growth and proliferation of SW579 cell lines by BRAF may be functioned by de-activating MEK/ERK signal pathway.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Thyroid Neoplasms
/
MAP Kinase Signaling System
/
RNA, Small Interfering
/
RNA Interference
/
Cell Line, Tumor
/
Proto-Oncogene Proteins B-raf
/
Cell Proliferation
/
Genetics
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Journal of Central South University(Medical Sciences)
Year:
2013
Type:
Article
Similar
MEDLINE
...
LILACS
LIS