Effect of calcitonin gene-related peptide on isoprenaline-induced cardiac fibroblast proliferation and collagen expression / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 545-552, 2013.
Article
in English
| WPRIM
| ID: wpr-814845
ABSTRACT
OBJECTIVE@#To explore the inhibitory effect of calcitonin gene-related peptide (CGRP) on cardiac fibroblast proliferation and collagen synthesis induced by isoprenaline and the underlying mechanism.@*METHODS@#The primary cultured cardiac fibroblasts were incubated with isoprenaline (10(-5) mol/L) for 48 h after pretreatment with CGRP (10(-8) or 10(-7) mol/L) for 1 h. Cell activity was detected by MTT. The mRNA expression of collagen (types I and III) and connective tissue growth factor (CTGF) was determined by RT-PCR, and the levels of intracellular ROS were determined by DCFH-DA fluorescent probe.@*RESULTS@#Isoprenaline significantly promoted fibroblast proliferation and up-regulated collagen (types I and III) and CTGF mRNA expression concomitantly with an increase in ROS production, which were attenuated by CGRP. The effect of CGRP on cardiac fibroblasts was inhibited by CGRP8-37, a selective antagonist of CGRP receptor.@*CONCLUSION@#CGRP is able to protect cardiac fibroblasts against isoprenaline-induced proliferation and collagen expression, which might be related to the down-regulation of CTGF expression through inhibition of ROS production.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
RNA, Messenger
/
Calcitonin Gene-Related Peptide
/
Cells, Cultured
/
Reactive Oxygen Species
/
Rats, Sprague-Dawley
/
Cell Biology
/
Collagen Type I
/
Collagen Type III
/
Myocytes, Cardiac
Limits:
Animals
Language:
English
Journal:
Journal of Central South University(Medical Sciences)
Year:
2013
Type:
Article
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