Preliminary screen of metastasis-related genes in cervical squamaous cell carcinoma / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 405-412, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-814866
ABSTRACT
OBJECTIVE@#To explore the differentially expressed gene profile in cervical squamous cell carcinoma tissues with and without pelvic lymph node metastasis by microarray technique and to screen tumor metastasis-related genes.@*METHODS@#Cancer tissue samples were collected from 4 cervical squamous cell carcinoma patients with pelvic lymph metastasis and 6 patients without metastasis before the treatment. Differentially expressed gene profile was identified between cervical squamous cell carcinoma tissues with and without pelvic lymph node metastasis by NimbleGen microarray. Fluorescent real-time quantitative PCR was employed to validate the accuracy of the microarray by detecting the expression of genes picked from the differentially expressed gene profile. We screened tumor metastasis-related genes through the gene bank, PUBMED and gene ontology analysis.@*RESULTS@#With the application of microarray, 329 genes were identified as markedly differentially expressed genes between cervical squamous cell carcinoma tissues with and without pelvic lymph node metastasis. Real-time PCR was in accordance with microarray. Forty-four genes associated with tumor metastasis were identified from the differentially expressed gene profile.@*CONCLUSION@#Gene profile in cervical squamous cell carcinoma tissues associated with pelvic lymph node metastasis is preliminarily identified, indicating that lymph node metastasis of cervical squamous cell carcinoma is a process involving numerous genes.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Carcinoma, Squamous Cell
/
Gene Expression Regulation, Neoplastic
/
Uterine Cervical Neoplasms
/
Oligonucleotide Array Sequence Analysis
/
Gene Expression Profiling
/
Genetics
/
Lymphatic Metastasis
Limits:
Adult
/
Aged
/
Female
/
Humans
Language:
Chinese
Journal:
Journal of Central South University(Medical Sciences)
Year:
2013
Type:
Article
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