Combination of triptolide with sodium cantharidinate synergistically enhances apoptosis on
hepatoma cell line 7721 / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 911-917, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-814944
ABSTRACT
OBJECTIVE@#To determine the combined cytotoxic effect and the molecular basis of triptolide and sodium cantharidinate on hepatoma cell line 7721.
@*METHODS@#After treating the hepatoma cell line 7721 with triptolide(9, 18, or 36 μg/mL) and/or sodium cantharidinate (2, 5, or 10 μg/mL), cell viability assay and apoptosis were examined by MTT and flocytometry, respectively. The protein levels of caspase 3 and nuclear factor κB were analyzed by Western blot.
@*RESULTS@#Viability of hepatoma cell line 7721 was inhibited by either the therapy of triptolide and/or sodium cantharidinate (P<0.05) in a time- and dose-dependent manner. The combined effects of both drugs were better than those of the single drug (P<0.05). The combined therapy down-regulated the expression of NF-κB p65 (P<0.05) while up-regulated the expression of caspase-3 (P<0.05).
@*CONCLUSION@#Triptolide and sodium cantharidinate exert a synergistic toxic effect on hepatoma cell line 7721, which is related to increasing capase-3 activity and suppression of NF- κB.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Phenanthrenes
/
Cantharidin
/
Down-Regulation
/
NF-kappa B
/
Apoptosis
/
Carcinoma, Hepatocellular
/
Cell Line, Tumor
/
Therapeutic Uses
/
Diterpenes
Limits:
Humans
Language:
Chinese
Journal:
Journal of Central South University(Medical Sciences)
Year:
2016
Type:
Article
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